expression plasmids pcmv6-entry-empty Search Results


pcmv6 empty vector  (OriGene)
97
OriGene pcmv6 empty vector
Pcmv6 Empty Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcmv6 empty vector/product/OriGene
Average 97 stars, based on 1 article reviews
pcmv6 empty vector - by Bioz Stars, 2026-02
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pcmv6 entry  (OriGene)
95
OriGene pcmv6 entry
Overexpression of FOXA1 downregulated laminin-332 genes (LAMA3, LAMB3, LAMC2) in a human BCa cell line with basal-squamous subtype. The day before transfection, 2×105 cells of 5637 bladder cancer cells were plated in 6 well plates. Next day, attached cells were transfected with 2.5 mg of the following plasmid constructs: <t>pCMV6-Entry</t> (CMV empty vector), pCMV6-FOXA1 using Lipofectamine 3000. After 48 hrs incubation, RNA was extracted for q-RT-PCR analysis. mRNA expression of FOXA1, LAMA3, LAMB3, and LAMC2 were compared between empty vector - overexpressed and FOXA1-overexpressed 5637 cell line. Data are expressed as the mean ± S.D. from three independent experiments. *P<0.05, **P<0.01, ****P<0.0001 (Student’s t test).
Pcmv6 Entry, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcmv6 entry/product/OriGene
Average 95 stars, based on 1 article reviews
pcmv6 entry - by Bioz Stars, 2026-02
95/100 stars
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plasmid #ps100001  (OriGene)
90
OriGene plasmid #ps100001
Overexpression of FOXA1 downregulated laminin-332 genes (LAMA3, LAMB3, LAMC2) in a human BCa cell line with basal-squamous subtype. The day before transfection, 2×105 cells of 5637 bladder cancer cells were plated in 6 well plates. Next day, attached cells were transfected with 2.5 mg of the following plasmid constructs: <t>pCMV6-Entry</t> (CMV empty vector), pCMV6-FOXA1 using Lipofectamine 3000. After 48 hrs incubation, RNA was extracted for q-RT-PCR analysis. mRNA expression of FOXA1, LAMA3, LAMB3, and LAMC2 were compared between empty vector - overexpressed and FOXA1-overexpressed 5637 cell line. Data are expressed as the mean ± S.D. from three independent experiments. *P<0.05, **P<0.01, ****P<0.0001 (Student’s t test).
Plasmid #Ps100001, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid #ps100001/product/OriGene
Average 90 stars, based on 1 article reviews
plasmid #ps100001 - by Bioz Stars, 2026-02
90/100 stars
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pcmv6-entry ps100001  (OriGene)
90
OriGene pcmv6-entry ps100001
Overexpression of FOXA1 downregulated laminin-332 genes (LAMA3, LAMB3, LAMC2) in a human BCa cell line with basal-squamous subtype. The day before transfection, 2×105 cells of 5637 bladder cancer cells were plated in 6 well plates. Next day, attached cells were transfected with 2.5 mg of the following plasmid constructs: <t>pCMV6-Entry</t> (CMV empty vector), pCMV6-FOXA1 using Lipofectamine 3000. After 48 hrs incubation, RNA was extracted for q-RT-PCR analysis. mRNA expression of FOXA1, LAMA3, LAMB3, and LAMC2 were compared between empty vector - overexpressed and FOXA1-overexpressed 5637 cell line. Data are expressed as the mean ± S.D. from three independent experiments. *P<0.05, **P<0.01, ****P<0.0001 (Student’s t test).
Pcmv6 Entry Ps100001, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcmv6-entry ps100001/product/OriGene
Average 90 stars, based on 1 article reviews
pcmv6-entry ps100001 - by Bioz Stars, 2026-02
90/100 stars
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empty control vector ps100010  (OriGene)
96
OriGene empty control vector ps100010
Overexpression of FOXA1 downregulated laminin-332 genes (LAMA3, LAMB3, LAMC2) in a human BCa cell line with basal-squamous subtype. The day before transfection, 2×105 cells of 5637 bladder cancer cells were plated in 6 well plates. Next day, attached cells were transfected with 2.5 mg of the following plasmid constructs: <t>pCMV6-Entry</t> (CMV empty vector), pCMV6-FOXA1 using Lipofectamine 3000. After 48 hrs incubation, RNA was extracted for q-RT-PCR analysis. mRNA expression of FOXA1, LAMA3, LAMB3, and LAMC2 were compared between empty vector - overexpressed and FOXA1-overexpressed 5637 cell line. Data are expressed as the mean ± S.D. from three independent experiments. *P<0.05, **P<0.01, ****P<0.0001 (Student’s t test).
Empty Control Vector Ps100010, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/empty control vector ps100010/product/OriGene
Average 96 stars, based on 1 article reviews
empty control vector ps100010 - by Bioz Stars, 2026-02
96/100 stars
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Image Search Results


Overexpression of FOXA1 downregulated laminin-332 genes (LAMA3, LAMB3, LAMC2) in a human BCa cell line with basal-squamous subtype. The day before transfection, 2×105 cells of 5637 bladder cancer cells were plated in 6 well plates. Next day, attached cells were transfected with 2.5 mg of the following plasmid constructs: pCMV6-Entry (CMV empty vector), pCMV6-FOXA1 using Lipofectamine 3000. After 48 hrs incubation, RNA was extracted for q-RT-PCR analysis. mRNA expression of FOXA1, LAMA3, LAMB3, and LAMC2 were compared between empty vector - overexpressed and FOXA1-overexpressed 5637 cell line. Data are expressed as the mean ± S.D. from three independent experiments. *P<0.05, **P<0.01, ****P<0.0001 (Student’s t test).

Journal: American Journal of Clinical and Experimental Urology

Article Title: Characterization of laminin-332 gene expression in molecular subtypes of human bladder cancer

doi:

Figure Lengend Snippet: Overexpression of FOXA1 downregulated laminin-332 genes (LAMA3, LAMB3, LAMC2) in a human BCa cell line with basal-squamous subtype. The day before transfection, 2×105 cells of 5637 bladder cancer cells were plated in 6 well plates. Next day, attached cells were transfected with 2.5 mg of the following plasmid constructs: pCMV6-Entry (CMV empty vector), pCMV6-FOXA1 using Lipofectamine 3000. After 48 hrs incubation, RNA was extracted for q-RT-PCR analysis. mRNA expression of FOXA1, LAMA3, LAMB3, and LAMC2 were compared between empty vector - overexpressed and FOXA1-overexpressed 5637 cell line. Data are expressed as the mean ± S.D. from three independent experiments. *P<0.05, **P<0.01, ****P<0.0001 (Student’s t test).

Article Snippet: On the following day, attached cells were transfected with 2.5 mg of the following plasmid constructs: pCMV6-Entry (CMV empty vector; Origene, Rockville, MD), pCMV6-FOXA1 (Origene; RC206045) using Lipofectamine 3000 (Life Technologies, Carlsbad, CA).

Techniques: Over Expression, Transfection, Plasmid Preparation, Construct, Incubation, Reverse Transcription Polymerase Chain Reaction, Expressing